Stephen Barker Turner originated the role of Charlie in Theresa Rebeck's The Scene at the 2006 Humana Festival for New American Plays, where he had created Alden Hammond in Gina Gionfriddo's After Ashley two seasons before. Turner has had a history with Theresa Rebeck's work (having worked on her plays Our House and The Novelist) with Charles Isherwood of The New York Times proclaiming that his Charlie was filled with "great gallows humor and sensitivity."

After starring (with Jeffrey Donovan) in 2000's Blair Witch 2, Turner has appeared in several independent films including We Pedal Uphill (2008, with his wife, actress Jenny Bacon), Satellite (2006 Tribeca Film Festival), The Warrior Class (2004, with Robert Vaughn and Jamey Sheridan), Cosmopolitan (directed by Nisha Ganatra, with Bollywood star Roshan Seth), Machiavelli Rises, the short The Disappearance of Andy Waxman (with Rain Phoenix), and Matt Ross' (HBO's Big Love) Human Resources, among others.

Turner has also made appearances on television as everything from a political operative for the Christian right to a lawyer that keeps sex slaves in his basement, in such shows as Hack, Sex and the City, Swift Justice and every series in the Law and Order franchise.

Turner has had a long and varied career in the theater that includes the 50th anniversary production of Arthur Miller's All My Sons at the Roundabout Theatre (with John Cullum), Kevin Kline's Measure for Measure, As You Like It with Gwyneth Paltrow, and David Adjmi's new play The Evildoers.